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KMID : 0380020000150020195
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Korean Journal of Biotechnology and Bioengineering
2000 Volume.15 No. 2 p.195 ~ p.199
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Characteristics of Linoleic Acid Production by Marine Fungi in Sea Water Media
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Kim Su-Jung
Park Kyung-Won
Hur Byung-Ki
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Abstract
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Studies were made on the optimization of media to cultivate Thraustochytrium aureum ATCC 34304 for the enhanced
production of linoleic acid. The medium optimization was made with the artificial sea water medium. Yeast extract, sodium glutamate, peptone and tryptone were considered as nitrogen source. The effect of concentration of nitrogen source as well as initial glucose on the production of linoleic acid were investigated to optimize the media. The maximum yield of lipid was 0.302 mg/g cell mass when initial glucose concentration was 10 g/L and sodium glutamate was used as nitrogen source, and the yield of linoleic acid to unit cell mass was also maximum to be 8 % in that case. The highest linoleic acid concentration was obtained in the initial glucose concentration 30 g/L regardless of the kinds of nitrogen source and the linoleic acid concentration was 0.208 g/L when peptone was supplemented to be 2 g/L.
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KEYWORD
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Omega-6 fatty acid, linoleic acid, polyunsatruated fatty acid, artificial sea water
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